Field of Research: Analytical Chemistry
Name of author) (s): Fuad Al-Rimawi and Imad Odeh
Title of published work: “Development and Validation of an HPLC-UV Method for Determination of Eight Phenolic Compounds in Date Palms”
Name of Journal: Journal of AOAC International
Year: 2015
Volume: 98 (5)
Pages: 1335-1939
Publisher: J AOAC
Abstract:
A simple, precise, accurate, and selective method was developed and validated for determination of eight phenolic compounds (gallic acid, p-hydroxybenzoic acid, vanilic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, and sinapic acid) in date palms. Separation was achieved on an RP C18 column using the mobile phase methanol-water with 2% acetic acid (18 + 82, v/v). This method was validated according to the requirements for new methods, which include accuracy, precision, selectivity, robustness, LOD, LOQ, linearity, and range. The method demonstrated good linearity over the range 1-1000 ppm of gallic acid, p-hydroxybenzoic acid, vanilic acid, caffeic acid, and syringic acid with r² greater than 0.99, and in the range of 3-1000 ppm for p-coumaric acid, ferulic acid, and sinapic acid with r² greater than 0.99. The recovery of the eight phenolic compounds ranged from 97.1 to 102.2%. The method is selective because adjacent peaks of phenolic compounds were well separated with good resolution. The degree of reproducibility of the results obtained as a result of small deliberate variations in the method parameters and by changing analytical operators proved that the method is robust and rugged.
Contact author (s):
Name: Fuad Al‑Rimawi, PhD.
Address: Department of Chemistry, Al-Quds University
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
