Field of Research: Food and Nutrition
Name of author) (s): Ibrahim Abbasi, Al-Shareef Abdel-Kareem, Matouk Imad, Akkawi Muataz
Title of published work: “Development of a PCR/RLB Test for Meat Source Authentication in Processed Meat and Meat Products”
Name of Journal: Turkish Journal of Agriculture –Food Science and Technology
Year: 2015
Volume: 3 (4)
Pages: 216-220
Publisher: DOAJ
Abstract:
Concern over food authenticity has increased as a result of an increase in the consumption of processed foods containing meat or animal products. This raises a number of issues where the presence of pork in such foods is considered unacceptable in most Muslim and Jewish communities around the world. It also applied to the prohibition of beef consumption among Hindus. In order to ensure the absence of unwished meat products or mixing of meats from different sources in processed foods, a specific and sensitive test is essential. For this purpose we developed a molecular test based on DNA amplification by polymerase chain reaction (PCR) of the cytochrome b gene followed by reverse line blot analysis (RLB). Using this method many samples may be treated simultaneously and meat origins can easily be detected from processed foods or foods containing mixed meat sources; also, added pork components such as fat may be identified by this methodology. The PCR/RLB method is considered to be a sensitive and specific technique; it can detect one nucleotide change within the PCR-amplified DNA segment.
Keywords:
Meat source identification; PCR; RLB; DNA; Food
Contact info of the contact author (s):
Name: Ibrahim Abbasi, PhD.
Address: Department of Biology, Faculty of Science & Technology, Al-Quds University
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
