Location: Al Abraj/ Building B/ Sixth floor- Faculty of Medicine
Introduction:
The Microbiology Research laboratory at Al-Quds University was established in November 2012 from a DFG grant for 2011- 2013, the grant was extended another three years till 2016 and for another year without financial obligation till 2017.
The Microbiology Research Laboratory is focused on the ecology and virulence of Legionella species in water distribution systems in the West Bank and identification of Legionella pneumophila from lower respiratory tract specimens obtained from suspected pneumonia patients presenting at Makassed Hospital.
Machines:
# |
Machines |
# |
Manufacturer |
Application |
1 |
Autoclave |
1 |
Tuttnauer 3870 |
Sterilize equipment and supplies. |
2 |
Refrigerated Centrifuge 1.5-2 ml with 0.2 ml tubes rotor |
2 |
Thermo Scientific |
Spins small volume liquid samples at high speed at various temperatures. |
Heraeus |
||||
3 |
CO2 incubator |
1 |
Esco |
Cell cultures, tissue cultures and bacteria cultures. |
4 |
Computer |
3 |
Hp i3 |
|
Hpi2 |
||||
Pentium 4 |
||||
5 |
Deep freeze |
1 |
Panasonic |
Store and preserve bacteria for a longer period of time |
6 |
Digital balance |
1 |
RADWAG |
Measure accurate masses to prepare media and solution. |
7 |
Dry bath incubator 15ml +(1.5-2)ml |
1 |
MRC |
Provides a controlled dry heat environment for test tubes containing and controlled time. |
8 |
Filtration unit |
2 |
|
Filter water samples. |
9 |
Freezer- fridge |
2 |
LG |
Preserve samples, kits and reagents. |
Samsung |
||||
10 |
Gel documentation system-built in computer |
1 |
SYNGENE |
Imaging and documentation of PCR product suspended within agarose gels. |
11 |
Gel electrophoresis unit |
3 |
Biocom |
Separation and analysis of PCR products. |
Biometra |
||||
12 |
Hot plate |
1 |
Fried electric |
Heat and stirrer liquid solution |
13 |
Incubator
|
1 |
Heraeus |
Maintains optimal temperature to grow bacterial culture.
|
14 |
Micro plate reader |
1 |
|
Quantification of nucleic acid, protein , enzyme activity assays or other biological, chemical and physical events of samples |
15 |
Microwave |
1 |
LG |
Prepare agarose for electrophoresis |
16 |
Nanodrop |
1 |
Thermo Scientific |
Measuring Nucleic acid and protein concentration |
17 |
pH meter |
1 |
MRC |
Measuring PH, conductivity and salt concentration of water samples and other solutions |
18 |
Pipette holder |
2 |
|
|
19 |
Power supply |
3 |
Biocom CS300V |
Supplies electric power to electrophoresis unit |
20 |
Printer |
2 |
20 |
Printer |
21 |
Real time PCR detection system |
1 |
Biorad CFX 96 |
Amplify and quantify DNA |
22 |
Ice maker |
1 |
Star line |
Making ice used in preparation reagents for PCR. |
23 |
Thermal cycler |
1 |
Biorad T100 |
DNA amplification. |
24 |
Thermometers |
2 |
|
|
25 |
UV transillumenator |
1 |
MRC |
View PCR product (DNA or RNA) that has been separated by electrophoresis through an agarose gel. |
26 |
UV hood |
1 |
Esco / class 11 |
Provide sterile condition for media preparation and PCR reagent preparation. |
Biobase |
||||
27 |
Vacuum pump |
2 |
KNF Lab |
Increase the rate of filtration. |
Lab Tech |
||||
28 |
Vortex |
2 |
MRC |
Mix liquid samples. |
Labnet |
||||
29 |
Water bath
|
1 |
Lab Tech |
Provide optimal temperature for the reaction . |
30 |
Distillator |
1 |
GFL 2004 |
Provide distilled water |
31 |
UV/VIS Spectrophotometer |
1 |
Jenway 6715 |
Quantitative measurement for absorbance , transmittance and concentration for samples |
32 |
Refrigerated Centrifuge 15- 50ml with 96 well plate |
1 |
Centurion scientific |
Spins large volume of liquid samples at high speed at various temperature |
33 |
UBS |
2 |
3KVA |
|
34 |
SSCP Gel electrophoresis unit with power supply |
1 |
Eitan |
Discover new DNA polymorphisms apart from DNA sequencing |
Pik lab |
||||
35 |
Pipette filler |
2 |
Accumax |
|
36 |
Pipette 0.5-10 µl |
3 |
Eppendrof research plus |
|
37 |
Pipette 2-20 µl |
1 |
Eppendrof research plus |
|
38 |
Pipette 5-50 µl |
1 |
Win lab |
|
39 |
Pipette 20-200 µl |
3 |
Eppendrof research plus |
|
40 |
Pipette 100-1000 µl |
4 |
Eppendrof research plus |
|
41 |
Pipette 0.5- 5 ml |
1 |
Spin react |
|
|
|
|
Eppendrof research plus |
|
42 |
Pipette 10-100 µl |
1 |
Microlit |
|
43 |
Pipette 1-10 µl |
1 |
Biopette Labnet |
|
44 |
Pipette 50-300 µl |
1 |
XL3000iTM |
|
45 |
Fluorescent microscope |
1 |
OLYMPUS (BX53) |
|
46 |
Inverted microscope |
1 |
OLYMPUS (CKX31) |
|
47 |
Bench top shaking incubator |
1 |
Labnet |
|
48 |
CO2 cylinder 25Kg with regulator |
|
|
Regulate CO2 level in the CO2 incubator |
49 |
Liquid nitrogen tank |
1 |
|
|
50 |
Air condition
|
2 |
Tadiran |
|
51 |
DNA extraction hood |
1 |
|
|
Director: Dina M. Bitar PhD/ Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Master student:
- Mutasem Burghal.