Research Labs

Medical Sciences Research Lab

Sameer A. Barghouthi

A. This research laboratory mainly applies:

  • DNA analyses including DNA/RNA extraction and PCR.
  • Bacterial identification based on DNA sequencing (using commercial services). 
  • Antibiotic research focusing on antibacterial agents produced by Streptomyces spp.

Location: Health Complex Building (Hc009)

B. Master and BSc students under advisors’ supervision have been using the lab for training and for their research projects.

C. List of available equipment and instrument:

  • Shaking water bath.
  • Nodding shaker
  • Hot air incubator
  • Water jacketed CO2 incubator
  • Weighing scale (four-decimal digits)
  • Autoclave
  • Biological safety cabinet.
  • Microcentrifuge
  • Low speed centrifuge (15-ml tubes).
  • UV-illuminator box for gel viewing.
  • Hand held UV-lamp
  • DC-Electric power supplier
  • Agarose gel electrophoresis unit
  • Heat sealer
  • Gel- vacuum dryer
  • Filter sterilizing unit (liquid filtration)
  • Bright light viewing box
  • Compound microscope (4x, 10x, 45x, 100xOil)
  • Dissecting microscope (1 to 7x zoom)
  • Anaerobic / candle jar.


  • This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Mobile: 0599379561
  • Department of Medical Laboratory Sciences,  Faculty of Health Professions, Al-Quds University


The MLSRL supports research in medical microbiology and molecular biology. Currently research is focused on antibacterial antimicrobial agents extracted from Streptomyces species isolated from soil samples. In addition, research areas of interest include bacterial resistance to antibiotics and Helicobacter pylori research. Other areas include isolation and classification of Bacillus spp., immunological aspects of Hepatitis B virus and others.  Collaboration with colleagues from local institutions; universities and medical laboratories, hospitals, and health care centers is a continuous process. Another important contribution that has been carried out in this research lab is student training in research; undergraduate students, graduation projects, and thesis projects for graduate MSc. Students.

The research areas covered include:

  1. Microbial studies including isolation, characterization and identification.
  2. Diagnostic microbiology.
  3. Problem solving research in respect to bacterial growth and identification.
  4. Isolation, identification, and evaluation of antimicrobial agents including comparative studies.
  5. Bacterial storage and maintenance.
  6. Molecular studies including human viruses and human genomics.
  7. Essential equipment include Biosafety biological hood, autoclave,  centrifuges, accurate balance/scale, shaking water bath, filter sterilization units, microscopes, incubators.

Publications contributed by this Laboratory:

  • Hamarsheh (2007) Genetic Variability of Sand Fly Phlebotomus papatasi Populations (Diptera: Psychodidae) Originating from the West Bank, Palestine. Journal of Entomology 4, 425-434.
  • Barghouthi S (2009) Helicobacter pylori: A theoretical background with a practical approach to growth and behavior (Vol I) Acknaton Design and Print, Dar Al-Shorouq, Ramallah, Palestine.
  • Patent: Universal Method for Bacterial Identification, PCR-sequencing based.
    Palestinian Authority Patent No. 146, 2009.  A method for the detection and identification of Bacteria.
  • Patent: Palestinian Authority Patent No. 147, 2009. The use of bacteria in biocontrol of the Parasitic plant Orobanche spp,
  • Barghouthi S and Salman M (2010) Bacterial Inhibition of Orobanche aegyptiaca Pers. and Orobanche cernua Loefl. Radical Elongation. Biocontrol Science and Technology 20: 423-435.
  • Barghouthi S  2011. A Universal Method for the Identification of Bacteria Based on General PCR Primers. Indian Journal of Microbiology. Indian Journal of Microbiology, 51:430-444. On line: DOI: 10.1007/s12088-011-0122-5
  • Barghouthi SA and Dima K. Al Zughayyar 2012. Detection of Neisseria meningitidis and unknown Gammaproteobacteria in cerebrospinal fluid using the two-step universal method.  [Abstract] [Full Article - PDF]  African Journal Microbiol Research. 6(14): 3415-3424. DOI: 10.5897/AJMR11.1213
  • Barghouthi SA, G. Hammad, M. Kurdi : Acinetobacterlwoffii Induced Cellulitis with Allergy-like Symptoms. The Internet Journal of Microbiology. 2012 Volume 10 Number 2.
  • Salman M, Abuamsha R  and  Barghouthi SA (2013). Interaction of Fluorescent Pseudomonads with Pythium ultimum and Rhizoctonia solani in Cucumber Roots. American Journal of Experimental Agriculture 3(1): 240-251.
  • Barghouthi SA and Khalid Younis (2014). First time isolation of Pseudomonas argentinensis from human skin infection. J Microbiol Infectious Diseases 04: 78-83.
    doi: 10.5799/ahinjs.02.2014.02.0133.
  • Abu Hilu R, Dudeen O, Barghouthi SA. (2014)  Association between Helicobacter pylori Infection and Vitamin B12 Level Among Palestinian People in Hebron, Palestine. Accepted ; Turkish journal of Medical Sciences.
  • Warad I., Ali O., Ahed R., Bani Odeh A., Barghouthi S.A., Naveen S., Elmsellem H., Daraghmeh I., Lokanath N. K., Allali M.,. 2017. Synthesis, physicochemical, conformation and quantum calculation of novel N- (1-(4-bromothiophen-2-yl)ethylidene)-2-(piperazin-1-yl)ethanamine Schiff base. J. Materials and Environmental Science 8(11):3844
  • Ala'a Janim1, Mohammed Al-Nuri1, Abdallah Bani Odeh2, Sameer A. Barghouthi2, Mohammad Ayesh2, Anas Al Ali1, Sameer Amereih3, Ismail Warad (2016) TD-DFT and DFT studies on geometry, spectral, thermal and NMR of N'-(di(pyridin-2-yl)methylene)-2-hydroxybenzohydrazide. J. Mater. Environ. Sci. 7 (9) (2016) 3447-3453
  • Mazen Salman, Nabil Shahin, Nawaf Abu-Khalaf, Mohammad Jawabrih, Basima Abu Rumaileh, Ruba Abuamsha, Sameer A. Barghouthi. (2017). Antagonistic Activity of Pseudomonas Fluorescens Against Fusarium Oxysporum f. sp. Nievum Isolated from Soil Samples in Palestine. Journal of Plant Studies 6(2):1-8.  DOI:10.5539/jps.v6n2p1
  • Barghouthi SA, Ayyad I, Ayesh M, Abu-Lafi S (2017) Isolation, Identification, and Characterization of the Novel Antibacterial Agent Methoxyphenyl-Oxime from Streptomyces pratensis QUBC97 Isolate. J Antibiot Res 1(1): 105
  • Abdallah Bani Odeh, Issa Shtayeh, Mohammed Ayesh, Ismail Warad and Sameer A. Barghouthi. 2017. General Purpose Media (BNO) for Growing Fastidious Gram Negative (FGN) Bacteria. Journal of Advances in Medicine and Medical Research, ISSN: 2456-8899, ISSN: 2231-0614 (Past),Vol.: 22, Issue.: 6 . DOI : 10.9734/JAMMR/2017/34450
  • Barghouthi SA. 2017. The Epimmunity Theory: The Single Cell Defenses against Infectious and Genetic Diseases. Front Immunol. 2017; 8: 694.  doi:  10.3389/fimmu.2017.00694PMCID: PMC5468598
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